NAMs in Neuroscience

Brain organoids, blood-brain barrier models, and human-relevant approaches to understanding neurological diseases—because the human brain is unlike any other species

99%
Alzheimer's drugs fail clinical trials
98%
BBB blocks drug delivery to brain
86B
Neurons in the human brain
$600B+
Annual cost of neurological disorders

The Neuroscience Translation Problem

The human brain is the most complex organ, with unique cell types, connectivity patterns, and disease mechanisms not found in other species. Mouse models have consistently failed to predict human responses in neurological diseases—particularly Alzheimer's, where over 200 drugs that worked in mice have failed in human trials. Human brain models are essential.

The Blood-Brain Barrier Challenge
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Blood Side

Drugs circulate in the bloodstream but cannot freely enter the brain

🧱

Blood-Brain Barrier (BBB)

Tight junctions between endothelial cells block 98% of small molecules and nearly all large molecules

🧠

Brain Side

Protected environment where neurons require precise drug concentrations

Human BBB models are critical because mouse BBB has different transporter expression and permeability

Human Brain Models

NAMs that capture human-specific neurobiology

Brain Organoids (Cerebral Organoids)

3D structures grown from human iPSCs that self-organize into brain-like tissue with distinct regions and cell types.

  • Multiple brain cell types
  • Spontaneous electrical activity
  • Models neurodevelopment
  • Patient-derived disease models

BBB-on-Chip

Microfluidic devices that recreate the blood-brain barrier with human endothelial cells, pericytes, and astrocytes.

  • Tests drug BBB penetration
  • Flow conditions like blood vessels
  • Human transporter proteins
  • Disease-state modeling

Neural Spheroids

Smaller, simpler 3D neural cultures for high-throughput screening of neurotoxicity and drug effects.

  • Rapid, scalable production
  • Neurotoxicity screening
  • Synapse formation
  • Cost-effective

Multi-Electrode Arrays (MEAs)

Platforms that record electrical activity from human neurons to study neural network function and drug effects.

  • Real-time activity monitoring
  • Seizure liability detection
  • Network connectivity analysis
  • Functional readouts

Neurological Disease Modeling

Using human cells to understand brain diseases

Alzheimer's Disease

Model amyloid plaques and tau tangles in human neurons that carry patient mutations (APP, PSEN1).

NAM: Patient-derived brain organoids with familial AD mutations

Parkinson's Disease

Study dopaminergic neuron degeneration using iPSC-derived midbrain organoids from PD patients.

NAM: Midbrain organoids with alpha-synuclein pathology

ALS (Lou Gehrig's Disease)

Investigate motor neuron death using human motor neurons derived from ALS patient cells.

NAM: iPSC-derived motor neurons with SOD1 mutations

Epilepsy

Study seizure activity using human neural networks on MEAs and brain organoids.

NAM: MEA recordings from patient-derived neurons

Brain Tumors (Glioblastoma)

Test drugs on patient-derived glioblastoma organoids that retain tumor heterogeneity.

NAM: Patient-derived tumor organoids in brain tissue co-culture

Multiple Sclerosis

Model demyelination and immune attack on oligodendrocytes using human cell systems.

NAM: Human oligodendrocyte-neuron co-cultures

Human vs. Mouse Brain

Critical differences that affect drug development

Feature
Human
Mouse
Cortical neurons
16 billion
14 million
Cortical folding (gyrification)
✓ Highly folded
✗ Smooth (lissencephalic)
Human-specific cell types
✓ Present
✗ Absent
BBB transporter expression
Human pattern
Different pattern
Alzheimer's pathology
✓ Natural plaques/tangles
✗ Requires artificial transgenes
Prefrontal cortex development
✓ Highly developed
~ Rudimentary