FDA ISTAND VALIDATED 87% Sensitivity 100% Specificity
Hepatotoxicity Research

Liver Toxicity Testing

DILI Prediction with Next-Generation Models

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87%
Sensitivity
DILI detection
100%
Specificity
False positive rate
#1
Cause
Of drug withdrawals
Sept
2024
FDA ISTAND accept

THE DILI CHALLENGE

Drug-induced liver injury (DILI) is the leading cause of acute liver failure and the primary reason for drug withdrawals from market. Traditional preclinical models fail to predict approximately 50% of clinical hepatotoxicity, representing a critical gap in drug safety assessment that organ-chips and liver organoids are now addressing.

FDA ISTAND ACCEPTANCE

REGULATORY MILESTONE
Emulate Liver-Chip: First Organ-Chip FDA Qualification

In September 2024, the FDA accepted Emulate's Liver-Chip into the Innovative Science and Technology Approaches for New Drugs (ISTAND) Pilot Program. Validation studies demonstrated 87% sensitivity for detecting compounds that cause DILI in humans, with 100% specificity (no false positives). This represents a significant advancement over traditional hepatocyte cultures and animal models.

Source: FDA ISTAND Pilot Program, Emulate press release September 2024

TECHNOLOGY COMPARISON

LIVER-ON-CHIP
Microfluidic Liver Models

Liver-chips incorporate primary human hepatocytes with liver sinusoidal endothelial cells, Kupffer cells, and stellate cells under physiological flow conditions. This enables assessment of bile transport, immune-mediated hepatotoxicity, and metabolic drug interactions.

LIVER ORGANOIDS
3D Hepatic Structures

Liver organoids derived from hepatic progenitors or iPSCs provide patient-specific models for hepatotoxicity screening. They express key drug-metabolizing enzymes (CYP450) and can be maintained for extended culture periods for chronic toxicity studies.

ADME-TOX APPLICATIONS

Beyond DILI prediction, liver models support comprehensive ADME-Tox assessment:

A
Absorption
First-pass metabolism
D
Distribution
Protein binding
M
Metabolism
CYP450 profiling
E
Excretion
Biliary transport

KEY TECHNOLOGY PROVIDERS

Organ-Chip

Emulate

FDA ISTAND validated Liver-Chip platform

MPS

CN Bio

PhysioMimix liver models for DILI

Spheroids

InSphero

3D liver microtissues for toxicity

PRIMARY SOURCES

← Clinical Trials in a Dish Next: Brain Organoids →